Figure 9. The presence of hMARCO restores TDM responses to a non-responsive cell line.

RAW264.7 cells that stably expresses human MARCO (hMARCO- RAW) and a control cell line expressing the empty vector (vector-RAW) were created (B). After 24 hours of stimulation with 3 µm diameter TDM-coated microspheres, the vector-RAW cell line did not produce TNF-α, however there was a significantly increased level of TNF-α production in the hMARCO expressing cell line (A, P<0.025). One representative experiment of 3 shown. Bars represent the mean and error bars represent the standard deviation of the mean.