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. 2009 Apr 30;11(2):R59. doi: 10.1186/ar2682

Figure 3.

Figure 3

In vivo treatment with apigenin reduced nucleosome-specific Th1, Th17, and IgG autoantibody production. In vivo treatment with apigenin (20 mg/kg) for 2 months markedly reduced nucleosome-specific Th1 and Th17 responses and IgG autoantibody production ex vivo as compared with vehicle-treated SNF1 mice. (a) Splenocytes from apigenin- or vehicle-treated SNF1 mice were stimulated with nucleosomes and analyzed for Th1(left panel) and Th17 (right panel) responses by enzyme-linked immunosorbent spot assay. 'CD3' indicates results upon stimulation with optimal amount of anti-CD3 antibody (0.2 μg/mL). (b) IgG autoantibody levels of anti-dsDNA, anti-ssDNA, anti-nucleosome, and anti-histone in culture supernatants of lupus Th cell-B cell-nucleosome cocultures were analyzed by enzyme-linked immunosorbent assay. *P < 0.001, **P < 0.01, xP < 0.02, and +P < 0.05. dsDNA, double-stranded DNA; IL-17, interleukin-17; SNF1, (SWR × NZB)F1; ssDNA, single-stranded DNA; Th, T helper.