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. 2009 Feb 25;10(2):R24. doi: 10.1186/gb-2009-10-2-r24

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Copyright © 2009 Immink et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Analyses of MADS box protein interactions in protoplasts by FRET. Arabidopsis leaf protoplasts, co-expressing MADS box proteins fused to either CFP or YFP, were analyzed by FLIM, in order to detect FRET. One representative protoplast is shown for each analyzed combination. The left panels display the intensity channel, the middle panels show the fluorescence lifetime image of the same nucleus in a false color code, and the right panels depict histograms representing the distribution of fluorescence lifetime values over the nucleus. FLIM analysis on a protoplast transiently expressing (a) pECFP + PI-YFP (negative control); (b) SEP3-CFP + SEP3-YFP; (c) AP3-CFP + AP3-YFP; (d) PI-CFP + PI-YFP; (e) AP1-CFP + SEP3-YFP; (f) PI-CFP + AP3-YFP; (g) PI-CFP + AP3-YFP + SEP3. Scale bars = 10 μm.