Table 1.
SAGE library | Dorsal | Ventral | Total |
Sequenced colonies | 1,265 | 1,018 | 2,283 |
Repeated ditags | 2,183 (12.2%) | 359 (2.2%) | 2,542 (7.4%) |
Ditags* | 15,773 | 16,057 | 31,830 |
Tags† | 31,538 | 32,104 | 63,642 |
Linker tags | 363 | 57 | 420 |
Total experimental tags | 31,175 | 32,047 | 63,222 |
Unique experimental tags | 14,546 | 14,486 | 23,766 |
Experimental tags matching to the genome | 14,352 | 14,347 | 23,455 |
SAGE libraries were prepared from total RNA of dorsal and ventral explants of X. tropicalis gastrula. Concatemer sequences were processed for tag extraction and comparison between libraries. *Repeated ditags were considered only once. †Tags including 'N' in the sequence were not considered (eight tags in the dorsal library and ten tags in the ventral library).