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. 2009 Jun;4(6):539–541. doi: 10.4161/psb.4.6.8697

Figure 1.

Figure 1

Generation of ROS by Pto DC3000 bacterial extracts in cerk1 mutant plants. (A) ROS burst in Arabidopsis Col-0 fls2/efr and Col-0 fls2/efr/cerk1 plants after treatment with 20 µl/ml of crude bacterial extracts from Pto DC3000. Crude bacterial extracts were prepared by boiling bacterial suspensions for 10 minutes and removing cell debris by centrifugation. Error bars indicate standard error of the mean (SEM). The results shown are representative of four independent experiments. (B) ROS burst in Arabidopsis Col-0 and Col-0 cerk1 plants upon treatment with water, 100 µg/ml chitin, or 30 µl/ml of Pto DC3000 peptidoglycan (PGN). Error bars indicate SEM. Similar results were obtained in two independent experiments.