Figure 2.

Quantitative PCR verification of three genes identified by microarray as differentially regulated in anergic B cells in the B6 versus MRL backgrounds. Unstimulated B cells were isolated from transgenic and nontransgenic mice in both backgrounds and quantified by RT-PCR using pre-designed TaqMan probe/primer sets. Relative copy number was calculated after normalization to expression levels of GAPDH in each sample. Shown is the ratio of anergic:naive transcript levels in each background. Genes shown are Ptpn22, protein tyrosine phosphatase non-receptor type 22; Birc1f baculoviral IAP repeat-containing 1f, also known as Naip6, or neuronal apoptosis inhibitory protein 6; and Ap1gbp1, AP1 gamma binding protein 1.