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. 2008 Apr 18;3:269–286. doi: 10.4137/bmi.s585

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© 2008 by the authors

This article is published under the Creative Commons Attribution By licence. For further information go to: http://creativecommons.org/licenses/by/3.0.

PMC Copyright notice

Autologus CD8+T cells with DC showed higher cytolytic activity against BEC-2 target cells at a high effector-to-target ratio than against Bamb-2 target cells. Autologous CD8+T cells were co-cultured with M1-, M4- or TNF-α-primed DC loaded with EBV-derived peptide, expanded with 100 U/ml IL-2, and on day 10, re-stimulated with BEC-2 or Bamb-2 target cells at various effector-to-target ratios. After overnight culture, the IFN-γ concentration in the supernatant was measured by ELISA (A), and specific lysis was measured by ⁵¹Cr release assay (B). Data are the mean ± S.E.M. of five independent experiments. *P < 0.05 compared with immature DC.

Autologus CD8+T cells with DC showed higher cytolytic activity against BEC-2 target cells at a high effector-to-target ratio than against Bamb-2 target cells. Autologous CD8+T cells were co-cultured with M1-, M4- or TNF-α-primed DC loaded with EBV-derived peptide, expanded with 100 U/ml IL-2, and on day 10, re-stimulated with BEC-2 or Bamb-2 target cells at various effector-to-target ratios. After overnight culture, the IFN-γ concentration in the supernatant was measured by ELISA (A), and specific lysis was measured by ⁵¹Cr release assay (B). Data are the mean ± S.E.M. of five independent experiments. *P < 0.05 compared with immature DC.