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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Protein Expr Purif. 2009 Apr 17;67(1):41–47. doi: 10.1016/j.pep.2009.04.007

Figure 8.

Figure 8

Western blot analysis of ErbB2 expression in CHO and CHO-bcl-xL clones. Clones were selected from stable pools of CHO and CHO-bcl-xL cells transfected with pcDNA3.1/zeo_erbB2 and CHO-bcl-xL cells transfected with pcDNA3.1/zeo (empty vector, control). Equal total cellular amounts of 20 μg were loaded per lane to ensure that band intensities reflect relative expression levels. Membranes were probed with anti-ErbB2 and anti-Bcl-xL antibodies. All samples were run on the same Western blot; non-relevant lanes have been removed for clarity. The arrows on the left indicate where the corresponding molecular weight marker from the protein ladder ran.