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. 2009 May 18;4(5):e5587. doi: 10.1371/journal.pone.0005587

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A–C) Intramolecular FRET recorded with Vα_1CC. (D–F) Intermolecular FRET recorded with Vα_1C+α_1CC. Channels were co-expressed in COS1 cells with α₂δ and Rβ_1b (A and D), Rβ_2d (B and E) or Rβ₃ (C and F). Shown are representative of histograms calculated from single exemplary cells for donor/acceptor ratio (left column), FRET efficiency (middle column) and distance (right column). Relative frequency was calculated for total number of pixels in ROI as described in Methods. The red solid line is the best fit to a Gaussian distribution with indicated means for r _V-C and r _V∼C.