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. 2009 May 18;4(5):e5587. doi: 10.1371/journal.pone.0005587

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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The Vα_1CC subunit was co-expressed in COS1 cells with α₂δ and Rβ_2d (A) or Rβ₃ (B). Shown are histograms of donor/acceptor ratio (left column), FRET efficiency (middle column) and distance (right column) determined in the plasma membrane region of two representative COS1 cells. The red solid line is the best fit to a sum of two Gaussian distributions with indicated means (green dotted lines) for intramolecular (r _C-V) and intermolecular FRET (r _C∼V).