Fig. 1.

Comparison of egg white glycoproteins from pigeon, chicken, and two species of gull by lectin blotting and immunoblotting. Egg white glycoproteins from pigeon (PEW, 2.5 μg/lane), chicken (CEW, 2.5 μg/lane), and two species of gull (GEW-J and GEW-C, 5 μg/lane each) were heat-denatured with the sample buffer containing 3% SDS and 5% 2-mercaptoethanol, and separated by SDS–PAGE (12.5%). After electrophoresis, the proteins were transferred to PVDF membranes and stained with Coomassie Brilliant Blue R-250 (CBB), GS-I lectin, anti-P₁ mAb, Con A or RCA-I.