Table I.
Monosaccharide compositions of GEW-glycoproteins
| Contenta | |||
|---|---|---|---|
| Monosaccharide | GOM | GOT | GEW |
| GlcN | 381 (6.42) | 98.0 (6.53) | 245 (5.57) |
| Man | 178 (3) | 45.0 (3) | 132 (3) |
| Gal | 204 (3.44) | 40.1 (2.67) | 145 (3.30) |
| α-Galb | 12.8 (0.216) | 3.02 (0.201) | 10.7 (0.243) |
| [% of total Gal] | [6.27] | [7.53] | [7.38] |
| NeuAcb | 66.3 (1.12) | 2.30 (0.153) | 47.0 (1.07) |
Amounts of protein were measured by the BCA assay, and the compositions of monosaccharides were analyzed by HPAEC. GlcN, d-glucosamine; Man, d-mannose; Gal, d-galactose; NeuAc, N-acetylneuraminic acid; GOM, gull ovomucoid; GOT, gull ovotransferrin; GEW, gull egg white.
aThe results are expressed as nanomoles of sugar/mg of protein. The values in parentheses are based on setting the number of Man residues as three.
bTerminal α-galactosides and sialic acids were released from glycoproteins with green coffee bean α-galactosidase and neuraminidase from A. ureafaciens, respectively, and the released monosaccharides were measured with HPAEC.