Figure 3. Inefficient rearrangement of the Jβ1^σ allele.

A. Analyses of Jβ1^σ/σ (open) and Jβ1^ω/ω (solid) αβ T cell hybridomas. All hybridomas have one complete in-frame TCRβ allele. The fraction in which the other allele was un-rearranged (GL), in the DJβ (DJ) and in the VDJβ (VDJ) configurations are indicated. B. Analyses of Jβ1^σ/ω αβ T cell hybridomas. Shown is the fraction of Jβ1^σ (open) and Jβ1^ω (solid) alleles in the GL, DJβ and VDJβ configurations. C. Southern blot analysis of rearrangement of the Jβ1^σ and Jβ1^ω alleles in thymocytes (thy) from two Jβ1^σ/ω mice and Jβ1^ω/ω and Jβ1^σ/ω ES cells as a reference for maximal retention of the germline bands. Genomic DNA was digested with HindIII and probed with probe D or a Rag-2 probe as a DNA loading control. The bands resulting from the germline Jβ1^σ (σ) and Jβ1^ω (ω) alleles are indicated. The molecular weight markers are indicated. The percent retention of the germline Jβ1^σ and Jβ1^ω alleles in the Jβ1^σ/ω thymocytes was calculated based on the intensity of the specific band in Jβ1^σ/ω thymocytes relative to the same band in Jβ1^σ/ω ES cells corrected for DNA loading using the intensity of the Rag-2 band.