Figure 2.

LCs are superior to CD14⁺ DCs at inducing the proliferation of allogeneic naïve CD4⁺ T cells and the differentiation of Type 2 cytokines secreting CD4⁺ T cells

(A) Skin-LCs induce more robust proliferation of allogeneic naïve CD4⁺ T cells than do dermal CD1a⁺ DCs or dermal CD14⁺ DCs. [H³]-thymidine incorporation. One of four experiments.

(B) In vitro-generated LCs stimulate stronger proliferation of allogeneic naïve CD4⁺ T cells than do CD14⁺ DCs. One five experiments.

(C) Naive CD4⁺ T cells primed with skin LCs secrete more Type 2 cytokines than those primed with dermal DC subsets. Proliferating naïve CD4⁺ T cells, primed by skin mDC subsets, were sorted and re-stimulated with CD3/CD28 mAbs overnight followed by cytokine analysis. One of three experiments.

(D) Naive CD4⁺ T cells primed with in vitro-generated LCs secrete more Type 2 cytokines upon restimulation. One of five experiments.

(E) LCs promote T cell differentiation towards IFNγ^-IL-4⁺ Type 2 T cells. Naïve CD4⁺ T cells cultured for 6 d with each in vitro-generated mDC subsets were stimulated with PMA and ionomycin in the presence of monensin. Intracytoplasmic cytokines were analyzed using anti-IFNγ and anti-IL-4 mAbs (upper panel). Some CD4⁺ T cells were restimulated with the same DC subset for 3 days before the cytokine analysis (lower panel). One of ten experiments.