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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Immunity. 2008 Sep;29(3):497–510. doi: 10.1016/j.immuni.2008.07.013

Figure 7.

Figure 7

Both CD14+ DCs and LCs efficiently activate memory T cells

(A) Flu-MP58-66 peptide-loaded CD14+ DCs express more Flu-MP58-66/HLA-A201 complexes on the cell surface than peptide-loaded LCs, as determined with a Flu-MP58-66/HLA-A201-specific tetramerized monoclonal antibody.

(B) Frequency of Flu-MP58-66-specific CD8+ T cells analyzed with Flu-MP58-66-HLA-A201 tetramer 9 days after activation of CD8+ T cells with Flu-MP peptide-loaded LCs or CD14+ DCs from an HLA-A201+ donor.

(C) Kinetics of Flu-MP58-66-specific CD8+ T cell expansion in response to stimulation with peptide-loaded LCs or CD14+ DCs.

(D) CD14+ DCs efficiently stimulate autologous TT-specific memory CD4+ T cells. CD4+ T cells were stimulated with each autologous DC subset loaded with TT or none. At day 7, T cells were restimulated with CD34-DCs loaded with TT for 5 h, and intracytoplasmic IFNγ and IL-4 production was analyzed by flow cytometry. One of four experiments.