Figure 1.

Binding of DiO-labeled OxLDL lipid microemulsions to mouse macrophages in the presence of unlabeled native LDL lipids (■), OxLDL lipids (●), intact native LDL (□), or OxLDL (○). Elicited mouse macrophages were incubated in the presence of 10 μg/ml DiO-labeled OxLDL lipid microemulsions for 2 h at 4°C with or without competitors added (expressed as μg of phospholipid per ml for lipid microemulsions and μg of protein for intact LDL). The cells were then washed, scraped, and the mean fluorescence intensity of 30,000 events measured by using flow cytometry and cell quest software.