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. 2009 May 23;65(Pt 6):648–650. doi: 10.1107/S1744309109018405

Figure 2.

Figure 2

Human RIG-I (218–925)–18-mer dsRNA binding assay at pH 7.0 using an electrophoretic mobility shift assay. Human RIG-I proteins were mixed with 18-­mer dsRNA in 100 mM HEPES pH 7.0. After incubation at room teperature for 20 min, the mixture was applied onto a 15% TBE–polyacrylamide gel and stained with ethidium bromide. The lanes are 1 kb DNA marker, 18-mer dsRNA, RIG-I protein and 18-mer dsRNA and RIG-I mixtures with RIG-I:dsRNA molar ratios of 0.12, 0.25, 0.5, 1, 2 and 4, respectively.