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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Anal Biochem. 2009 Apr 9;390(1):74–78. doi: 10.1016/j.ab.2009.04.004

Figure 1.

Figure 1

A. Detection of nNOS expression in 293T/nNOS cells and WT 293T cells. B. Comparison of the cell proliferation rate of 293T/nNOS cells and WT 293T cells. The results are from three independent experiments and are expressed as mean ± S.D. C. Activation of nNOS in 293T/nNOS cells by A23187 (5 μM). Cells were treated with or without A23187 for indicated times, and the amount of nitrite in the culture media was quantified with the Griess reagent. 293T/nNOS cells were treated with (a) or without (b) A23187 and WT 293T cells were treated with (c) or without (d) A23187.