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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Mol Microbiol. 2008 Jun 28;69(4):954–967. doi: 10.1111/j.1365-2958.2008.06331.x

Fig. 3. Promoter activation by YvrI and YvrHa.

Fig. 3

A) activity of oxdC- and yvrI-lacZ fusions in the absence or presence of YvrI overexpression from an ectopically integrated copy (using a pSWEET-based plasmid integrated at amyE; see Supplementary Table S2). Induction was obtained by addition of 2% xylose for 60 min. Cells either carry the wildtype complement of yvrI and yvrHa genes (HB7717 and HB7716), a yvrI deletion (HB7732 and HB7728), a yvrHa deletion (HB7733 and HB7729) or a yvrI yvrHa deletion (HB7734 and HB7730). Note that measurements of the strong oxdC and weak yvrI promoter activities are reported using different scales. (B) oxdC promoter activation in a host strain carrying a yvrI-yvrHa deletion in its genomic locus requires co-expression from ectopically integrated yvrI and yvrHa genes (strain HB7759). Immunoblot detection demonstrates that neither the accumulation nor mobility of YvrI-FLAG is affected by co-expression with YvrHa.