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. Author manuscript; available in PMC: 2010 Apr 3.
Published in final edited form as: Cell. 2009 Apr 3;137(1):73–86. doi: 10.1016/j.cell.2009.01.051

Figure 2.

Figure 2

Twist-1 interacts with PGC-1α and suppresses its transcriptional activity. (A) Does-dependent inhibition of transcriptional activity of Gal4-PGC-1α by twist-1. (B and C) Twist-1 inhibits PGC-1α-mediated, but not agonist-mediated, activation of PPARs. PPARδ or PPARγ, 20 ng; PGC-1α, 20 ng; twist-1, 20 ng. (D and E) Interactions of twist-1 with co-activators. (F) PGC-1α interacts with the N-terminus of twist-1. (G) Twist-1 interacts with the C-terminal half of PGC-1α. (H) Suppression of PGC-1α activity by twist-1 requires their interaction. Left panel, Transcriptional activity of Gal4-PGC-1α fusion constructs was measured in the absence of twist-1. Right panel, the fold of transcriptional activity in the presence of twist-1 relative to the activity in the absence of twist-1 for Gal4-PGC-1α fusion constructs was measured.