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. Author manuscript; available in PMC: 2010 Apr 3.
Published in final edited form as: Cell. 2009 Apr 3;137(1):73–86. doi: 10.1016/j.cell.2009.01.051

Figure 3.

Figure 3

Twist-1 regulates PGC-1α-mediated mitochondrial oxidative metabolism and uncoupling. (A) Differentiated C2C12 muscle cells infected with PGC-1α and twist-1 adenovirus. (B) Twist-1 and PGC-1α interact at physiological levels in brown fat adipocytes. (C) Brown fat preadipocytes expressing a lentivirus twist-1 RNAi construct or control construct were infected with PGC-1α RNAi or control adenovirus during differentiation. (D) Gene expression in differentiated brown fat adipocytes infected with twist-1 knockdown adenovirus. (E) Mitochondrial DNA content indicated by the level of mitochondrial genome-encoded CoxII gene. (F) Left, representative electron microscopy micrographs from differentiated brown fat adipocytes. Right, Quantification of mitochondrial density from thirteen micrographs. M, mitochondria. (G) Gene expression in differentiated brown fat adipocytes infected with adenovirus expressing twist-1, PGC-1α, or both.