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. Author manuscript; available in PMC: 2010 Apr 3.
Published in final edited form as: Cell. 2009 Apr 3;137(1):73–86. doi: 10.1016/j.cell.2009.01.051

Figure 4.

Figure 4

Transgenic expressing twist-1 in adipose tissue inhibits brown fat metabolism. (A) Twist-1 transgene (TG) expression in the brown and white fat tissue. (B) Net body weight gain in wild-type control littermates (WT) and transgenic (TG) mice after a 2-month high fat diet (n=7–12 mice per group). Their initial body weights were similar, 16.65 ± 0.21 g (WT) vs. 16.80 ± 0.43 g (TG) (p=0.73). (C) Representatives of brown fat histology after a 7-week high fat diet. (D) Consumption of high fat diet (n=5 mice per group). (E) Gene expression in brown fat and white fat (n=6 mice per group). **p<0.01. (F) Ex vivo oxygen consumption of brown fat (n=4 mice per group). (G) Mitochondrial density. Left, representative electron microscopy micrographs. Right panel, quantification from twelve micrographs of four mice per group. M, mitochondria; L, lipid droplets. (H) Body temperature was recorded in a 10-min interval (n=8 mice per group). *p<0.05