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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Curr Opin Allergy Clin Immunol. 2008 Dec;8(6):547–552. doi: 10.1097/ACI.0b013e32831665d2

Figure 2. Schematic of H22-Fel d 1.

Figure 2

A) The humanized anti-CD64 monoclonal antibody, H22, was generated by CDR grafting from the mouse mAb M22. The humanized mAb contains 5–10% of the original mouse sequence. The sFv of H22 was linked to Fel d 1 by cloning and H22-Fel d 1 was produced in Baculovirus and Pichia pastoris expression systems [38, 40]. The sFv comprises the variable regions of heavy (VH) and light (VL) chains of H22 produced by joining VH and VL DNAs together with a linker to obtain a single DNA fragment for cloning and expression. B) H22-Fel d 1 binds to FcγRI outside the Fc binding region of the receptor.