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Induction of apoptosis by Smac mimetics in the human breast cancer MDA-MB-231 cell line. Cells were treated for 48 hours and apoptosis was determined using Annexin V-propidium iodide(P.I.) staining by flow cytometry. Annexin V (+) and P.I. (−) cells are labeled as early apoptotic cells, whereas Annexin V (+) and P.I. (+) cells are labeled as late apoptotic cells. Percentage of early and late apoptotic cells are shown.
