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. 2009 Apr 16;28(10):1505–1517. doi: 10.1038/emboj.2009.101

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Copyright © 2009, European Molecular Biology Organization

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits distribution, and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation or the creation of derivative works without specific permission.

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(A) PTEN^−/−MEFs were serum starved for 48 h and treated with H₂O₂ as indicated for 10 min. Protein lysates were analysed for pAkt^Ser473 as described before. (B) PTEN^−/−MEFs were infected with retrovirus expressing GFP–PTEN. After 5-day selection in puromycin (2 μg/ml), MEFs were serum starved, exposed for 10 min to H₂O₂ and analysed for Prdx1 oxidation and formation of dimeric structures by non-reducing SDS–PAGE. ^*Prdx1 hetero-dimer formation with other Prdxs. (C) PTEN^−/−MEFs were infected with retrovirus expressing shPrdx1, GFP–PTEN, H-Ras^V12 or various vectors carrying resistance gene only (pBabe, pMKO1). MEFs were plated in soft agar. Colonies were counted after 21 days. ^*P<0.001 (Student's t-test). (D) As under C, except MEFs express ErbB-2/neuT instead of H-ras^V12. ^**P<0.039 (Student's t-test). (E) Immunoblotting of MEFs from (C and D).