Table 1.
Prdx1−/−MEFs have a higher sensitivity to PI3K inhibitors in H-Ras or ErbB-2 induced transformation
| Wortmannin (nm) | H-Ras colony number in % | Ly294002 (μM) | H-Ras colony number in % | ErbB-2/neuT colony number in % | ||
|---|---|---|---|---|---|---|
| 0 | Prdx1−/− | 100±23.3 | 0 | Prdx1−/− | 100±11.2 | 100±4.6 |
| 5 | 96.3±5.7 | 10 | 38.3±15.2 | 47.3±6.6 | ||
| 10 | 97.8±13.5 | 25 | 5±10.1 | 3.2±4.1 | ||
| 0 | Prdx1+/+ | 100±4.1 | 0 | Prdx1+/+ | 100±11.2 | 100±4.6 |
| 5 | 55.9±12.5 | 10 | 26.3±5.5 | 25.1±6.6 | ||
| 10 | 32.7±24.4 | 25 | 4±8.7 | 3.2±4.8 | ||
| Prdx1−/−MEFs and Prdx1+/+MEFs were retrovirally infected with constructs carrying genes for H-RasV12 or ErbB-2/neuT (ErbB-2Val644Glu) and treated after plating in soft agar either with 10 or 25 μM Ly294002 every 2 days or Wortmannin 5 or 10 nM every day. Colonies were counted after 21 days. Decrease in colony formation (%) relates to untreated of the same genotype (%). This experiment is representative of three independent studies with two different sets of MEF clones from Prdx1 littermates. | ||||||