Figure 7.

Destabilization of ligand binding reduces desensitization. (A) Single subunit of -HERLK- showing glutamate bound in a cleft between D1 (blue) and D2 (orange). Interdomain salt bridge between Lys456 (D1) and Asp656 (D2) and also between Lys664 (D1) and Asp462 (D2) shown with σA-weighted 2mF_o DF_c electron density at 1σ. (B) Responses to 7-s applications of glutamate reveal an increase in steady-state response for KHERLK-AN compared with KHERLK-. (C) Bar plots showing the effect of K456A/D656N on the rate of desensitization (black) and recovery (grey) in KHERLK-.