Skip to main content
. 2009 Apr 4;28(10):1418–1428. doi: 10.1038/emboj.2009.88

Figure 4.

Figure 4

Promoter proximal enrichment of RNAPII (PPEP). (A) Histogram of the RNAPII occupancy ratio at promoter versus gene body. The distribution of the promoter/gene body ratio of all genes containing RNAPII (light blue) and of E2-responsive genes (red). The dashed lines represent the mean±1 × s.d. The 1228 genes display paused RNAPII of which only 21 are E2-responsive genes. (B) RNAPII occupancy profile. Genes were divided into bins relative to the transcription start site; −500 to −251, −250 to TSS, TSS to +250 and the remaining gene body was divided into four equal bins. For each group of genes, the mean number of tags per bin is plotted. E2-regulated genes on average have less RNAPII at their promoter regions as compared with the mean of all genes. Genes above the set threshold (mean+1 × s.d.) have a higher RNAPII occupancy at their promoter as compared with all genes and E2-regulated genes.