Figure 2.

Telomere sequencing reveals the defects of telomere replication in sua5Δ cells. (A) The analysis of Telomere I-L in wild type, first and fifth restreaked sua5Δ cells. Each column represents one sequenced telomere. The result of a representative clone of sua5Δ strain is shown here. (B) Statistics of the telomere sequencing results. The average point of divergence (APD) describes the average length of nondiverging telomere sequence, whereas the average length of divergence (ALD) stands for the average length of diverging telomere sequence. The results were summarized from two independent experiments shown in Supplementary Figure 3B. (C) Longer telomeres are not efficiently elongated in sua5Δ. The average lengths of the telomere extensions are determined for nondivergent telomeres that are longer or shorter than 170 nt in length. Telomeres shorter than 100 nt are excluded from the analysis because of the reported high recombination rate for short telomeres at TEL01L. The P-values associated with two-tailed unpaired t tests are shown. (D) Length of telomere extension as function of telomere length. The lengths of diverging regions are plotted as a function of the nondivergent sequence, which represents the original telomere sequence. (E) Frequency of telomere extension as function of telomere length. Telomere sequences are ordered according to the nondivergent sequence length and pooled into subgroups containing 10 telomeres each. The frequency of elongation in each subgroup is calculated and plotted as a function of telomere length.