Figure 3.

Short-term engraftment by c-myb knockdown bone marrow. Irradiated hosts were injected with 2.5 × 10⁶ test WT or KD total bone marrow cells together with 5 × 10⁵ competitor WT from CD45.1/CD45.2 animals (n=3). The percentages of the wild-type competitor and test donor populations are indicated. The ratios of wild-type competitor to test donor for all animals transplanted gave means and s.d. of 3±0.28, 6±1.4 and 3.2±0.15 for the peripheral blood (PB) from 4, 8 and 12 weeks, respectively. (A) Flow cytometric profiles of peripheral blood taken from primary engrafted animals 4, 8 and 12 weeks after transplantation with identifying host, competitor and KD donor cell populations based on CD45.1/CD45.2 staining. The competitor/donor ratio (C:D) is indicated in the top right corner of each histogram. (B) CD4, CD8, CD11b and B220 expression on peripheral blood cells of host, competitor and donor origin from primary engrafted animals 12 weeks after transplantation with WT or KD donor cells. (C) Blood smears from primary engrafted animals 6 months after transplantation with WT or KD donor cells. (D) Peripheral blood cells from primary engrafted animals 6 months after transplantation with WT or KD donor cells were cultured for 6 days. (E) Acetylcholine esterase staining (left panel) and analysis of CD41 and CD11b expression (right panel) in 6 day-cultured peripheral blood from a recipient transplanted with KD donor cells. (F) Methylcellulose culture of peripheral blood from a recipient transplanted with KD donor cells, showing a typical colony present after 2 weeks.