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Depletion of NAG induces a reduction in the expression level of p31. HeLa cells were transfected with lamin A/C siRNA, NAG (4160), or NAG (4382). At 72 h after transfection, the cells were solubilized in phosphate-buffered saline with 0.5% SDS, and the lysates (15 μg each) were separated by SDS-PAGE and analyzed by immunoblotting with the indicated antibodies.
