Table 1.
Spindle rotation and polar body extrusion
| Wild type | unc-116 | dhc-1(RNAi) | unc-116; dhc-1(RNAi) | |
|---|---|---|---|---|
| Rotation (MI) | ||||
| Rotation | 14 | 14 | 5 | 1 |
| Linear velocitya (μm/s) | 0.049 ± 0.008 (n = 8) | 0.053 ± 0.004 (n = 13) | 0.021 ± 0.002b (n = 3) | 0.055b (n = 1) |
| No rotation | 0 | 0 | 19 | 12 |
| Total | 14 | 14 | 24 | 13 |
| Rotation (MII) | ||||
| Rotation | 8 | 8 | 2 | 3 |
| Linear velocity (μm/s) | 0.052 ± 0.01 (n = 4) | 0.065 ± 0.005 (n = 5) | 0.046b (n = 1) | 0.074 ± 0.011b (n = 3) |
| No rotation | 0 | 0 | 13 | 7 |
| Total | 8 | 8 | 15 | 10 |
| Polar body extrusion (MI) | ||||
| Normal | 11 | 13 | 3 | 1 |
| Abnormal | 1 | 0 | 14 | 12 |
| Total | 12 | 13 | 17 | 13 |
| Polar body extrusion (MII) | ||||
| Normal | 5 | 8 | 1 | 0 |
| Abnormal | 1 | 0 | 14 | 10 |
| Total | 6 | 8 | 15 | 10 |
| Two MII spindles assemble | 0 (n = 8) | 0 (n = 8) | 3 (n = 15) | 5 (n = 10) |
Numbers indicate number of spindles for each genotype from time-lapse sequences of meiosis where parameters were measurable. MI, meiosis I; MII, meiosis II.
a Linear velocity indicates the velocity of one spindle pole moving toward a fixed point on the cortex during spindle rotation.
b Linear velocities taken from films where spindle did rotate after dhc-1 RNAi, velocities of films with no rotation are not included.