Skip to main content
. 2009 Jun 1;20(11):2744–2754. doi: 10.1091/mbc.E08-11-1092

Figure 3.

Figure 3.

Autophagy is induced in Hsp47−/− cells. (A) Western blot analysis of LC3 in Hsp47−/− cells in the presence or absence of lysosomal inhibitors. Note that LC3-II is the membrane bound form. (B) Quantification of LC3-II (n = 3). (C) Immunofluorescence staining of endogenous LC3. Bar, 10 μm. (D) Preembedding immunoelectron microscopic observation of LC3 localization in Hsp47−/− cells treated with E64d/pepstatin A. Gold-enhanced gold particles showing the presence of LC3 (arrows) are localized on the membrane of an autophagosome (an arrowhead) fusing with lysosome. Bar, 500 nm. (E) Preembedding immunoelectron microscopic observation of LC3 localization. GFP-LC3 was expressed in Hsp47−/− (KO-13) cells and analyzed with anti-GFP antibody. Bar, 200 nm. (F) Double staining of type I collagen and endogenous LC3 in Hsp47−/− (KO-13) cells cultured in the presence or absence of 100 nM bafilomycin A1 for 6 h. Bar, 10 μm.