Figure 7.
PKC ζ is required for structural reorganization of the KIF network. Cells were pretreated with either a myristoylated scrambled peptide (1 μM, 60 min; A and B) or a myristoylated PKC ζ peptide antagonist (1 μM, 60 min; C and D) and exposed to static control (A and C) or shear stress (30 dyn/cm2; 60 min; B and D; arrow in B indicates direction of flow) conditions. Cells were then fixed and processed for indirect immunofluorescence using anti-K18; representative confocal micrographs are shown. White scale bar, 10 μm.