Figure 9.

Recruitment of LKB1 to promoters. (A) Schematic representation of pS2, cathepsin D (Cat D), and c-myc promoters and control regions (cr). (B) ChIP analysis using anti-LKB1 antibody showing recruitment of LKB1 to the promoters of pS2, Cat D, and c-myc in the presence and absence of E2 treatment (100 nM) in MCF-7 cells. Control region (cr) of pS2(−3000 to −2700), Cat D (−2965 to −2577), and c-Myc (−2125 to −1950) (C) G361 cells lacking endogenous LKB1 and ERα expression were transfected with ERα, plus LKB1 or R304W expression plasmids followed by ChIP using anti-LKB1 antibody. (D) MCF7 cells were transfected with siLKB1#2 duplex, left untreated or treated with E2 (100 nM). Expression of ERα-responsive genes pS2, Cat D, and c-myc was determined by RT-PCR (left) and expressed relative to GAPDH as determined by densitometry (right). Results are representative of three separate experiments.