Figure 1.

Hepcidin mRNA levels in hepatocytes in primary culture treated with serum and serum plus human holotransferrin [(holoTf (h)] Hepcidin mRNA levels in primary hepatocytes was determined by northern blot analysis. Hepatocytes were treated, after a 42 h period of serum-free culture, with either 0% serum (control cells), 10% serum, 30 μM human holotransferrin, or both, for 24 h. Total liver RNA (20 μg) was subjected to electrophoresis, blotted, and hybridized with hepcidin and 18S-labeled probes. The experiment was performed at least three times and a representative result is shown.