Figure 4.

Sensorgrams for gp120 binding to the CD4i mAb 17b. mAb 17b was attached to the sensor surface, after which the indicated gp120 molecule (at equal concentrations), with or without prior incubation with saturating levels of sCD4, was applied to the flow cell. A 300-sec association was followed by a wash with running buffer for an additional 300 sec, during which dissociation was measured. Kinetic constants derived from linear transformations of the data are presented in Table 1.