Fig. 4.
Filopodia stabilization is dependent on PDZ ligand motif (TRL) and ERM. Quantification of microcolony associated with filopodia on 3T3 cells (A) or 3T3 cell transfected with wild-type (wt) ezrin or dominant-negative ezrin (ΔNter) (B) infected for 15 or 30 min with E2348/69Δmap overexpressing MapEPEC (A and B) or MapΔTRL (A). One hundred colonies were counted from five independent experiments. Results are presented as mean ± SD. Significant differences are indicated by asterisks (*P < 0.01). Expression of MapΔTRL had no effect at 15 min, but resulted in reduced filopodia at 30 min post infection (A). Map-induced filopodia is affected by expression of ezrin ΔNter at 30 min post infection (B). Wild-type ezrin, but not ezrin ΔNter, is recruited to the Map-induced filopodia (C); VSV-G-tagged ERM was stained with rabbit anti-VSV-G (Red) and DNA with Hoechst (Blue).