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. 2008 Oct 31;11(2):217–229. doi: 10.1111/j.1462-5822.2008.01248.x

Fig. 2.

Fig. 2

A. Fluorescence microscopy of serum-starved Swiss 3T3 cells uninfected or infected with wild-type E2348/69 or E2348/69 expressing EspT for 90 min. Actin was stained with Oregon green phalloidin and E2348/69 was detected with a rabbit O127 antibody. Distinctive lamellipodia, which have a strong leading edge, wide lamella and well defined microspikes and filopodia that extend beyond the cell boundary, were observed on cells infected with E2348/69 expressing EspT but not in the wild-type control. Both strains induced formation of actin-rich pedestals beneath adherent bacteria. B. Serum-starved HeLa cells infected with E2348/69 expressing EspT exhibited membrane ruffles whereas those infected with wild-type E2348/69 did not. Both strains formed actin pedestals. C. Quantification of Lamellipodia and membrane ruffles on Swiss 3T3 and HeLa cells, respectively, after 90 min infection with wild-type E2348/69 or E2348/69 expressing EspT. One hundred cells were counted in triplicate in three independent experiments. Results are displayed as mean ± SEM.