Fig. 7.
A. EspT-induced cytoskeletal rearrangements are not dependent on ELMO or Dock180. Swiss 3T3 were transfected with the dominant negative forms of ELMO and Dock180 18 h prior to infection with E2348/69 expressing EspT. Neither ELMOT625 nor Dock180ISP had any effect on lamellipodia induction by EspT. B. Quantification of lamellipodia or membrane ruffles in Swiss 3T3 cells transfected with dominant negative ELMOT625 or Dock180ISP and infected with E2348/69 expressing EspT or cotranfected with IpgB1 from S. flexneri as control. One hundred cells were counted in triplicate from three independent experiments. Results are displayed as mean ± SEM.