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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: Nature. 2008 Mar 20;452(7185):370–374. doi: 10.1038/nature06780

Figure 3. βTRCP targets REST during oncogenic transformation.

Figure 3

a, TLM-HMECs were transduced with control or GFP-βTRCP1-expressing retroviruses. Lysates were probed with antibodies against REST (upper panel), βTRCP (middle panel), or Vinculin (lower panel). b, Cells from a were analyzed for anchorage-independent colony formation. Assays were performed in quadruplicate (error bars +/− s.d.). Representative of 3 independent experiments is shown. c, HMECs were transduced with retroviruses expressing wild-type REST (REST-WT), degron-mutant REST (REST-MUT), and/or βTRCP1. Cell numbers were monitored for 4 days after plating on tissue-culture dishes. (open circles: vector-1+vector-2, closed circles: βTRCP1, open triangles: βTRCP1+vector-2, closed diamonds: βTRCP1+REST-WT, open squares: βTRCP1+REST-MUT) d, Cells from c were assessed for anchorage-independent colony formation. Assays were performed in triplicate (error bars +/− s.d.). Representative of 2 independent experiments is shown.