Table 1.
Development of polarized culture RPE
| Author | REP cell type |
Chamber | Medium | TER (Ω·cm2) | Features of differentiation |
|---|---|---|---|---|---|
| Geisen et al.15 | ARPE-19 | Transwell | DMEM/F12 (10%FBS) | <30 | Na-K ATPase, ZO-1, stress fiber |
| Geisen et al.15 | D407 | Transwell | DMEM-high glucose (5%FBS) DMEM-high glucose (20%FBS) |
<10 | ZO-1(-) |
| Geisen et al.15 | mRPE | Transwell | (3days)→DMEM-high glucose (10%FBS) |
<30 | ZO-1, stress fiber |
| Geisen et al.15 | hRPE | Transwell (human extracellular matrix coat) |
MEM (15 5%FBS) withN1supplement,nonessential amino acids, hydrocortisone, taurine and triiodo-thyronin |
>500 | ZO-1 |
| Hu et al.11 | hRPE | Millicell-PCF or Millicell-HA (both mouse laminin coat) |
Chee’s essential medium | >500 | Na-K ATPase, bestrophin |
| >700 | |||||
| Blaauwgeers et al.10 |
hRPE | Transwell (Matrigel coat) |
Iscove’s modified Dulbecco’s medium (1% normal human serum) |
<100 (3week) | ZO-1, TJ complexes , microvilli |
| Holtkamp et al.9 |
hRPE, ARPE-19 |
Transwell (Matrigel coat) |
Iscove’s modified Dulbecco’s medium (1% fetal calf serum) |
<20 (about 19day) |
ZO-1, cytokeratin 8/18, a glucose transporter protein, TJ complexes, microvilli |
| Dunn et al.13 | ARPE-19 | Ttranswell or Transwell-COL filters or Millicell CM or HA insert (Matrigel coat) |
DMEM/F12 (10%FBS) or specialized medium developed for tissue culture of human RPE.28 |
<40 DMEM/F12 <100(special medium) |
CRALBP, RPE65, pigment,TJ complexes, microvilli, polygonal shape |
| Maminishkis et al.12 |
hRPE | Transwell (human extracellular matrix coat) |
MEM (15-5%FBS) with N1 supplement, Glutamine-Penicillin-Streptomycin, Non essential amino acids, Taurine, Hydrocortisone, Triiodo-thyronin |
>500 | ZO-1, occludin, claudin, ezrin, CRALBP, RPE65, Na-K ATPase, pigmentation, TJ complexes, microvilli, basal infold |
| Kannan et al.14 | ARPE-19 | Transwell (laminin coat) |
MEM (10-1%FBS) with N1 supplement, Glutamine-Penicillin-Streptomycin, Non essential amino acids, Taurine, Hydrocortisone, Triiodo-thyronin |
<50 | ZO-1, occludin, NaK-ATPase (apical) |
| Ohno et al.20 | hRPE | T75 flask (laminin coat) |
DMEM (10%FBS), 10ng/ml bFGF | N/A | CRALBP |
| Ban et al.29 | Chick RPE | 12mm Transwell (laminin coat) |
SF2, culturing E14 neural retina in SF2 | <100 | ZO-1 |
| Sonoda et al.22 Sreekumar et al.24 |
hRPE | Transwell (fibronectin coat) |
MEM (15-1%FBS) with N1 supplement, Glutamine-Penicillin-Streptomycin, Non essential amino acids, Taurine, Hydrocortisone, Triiodo-thyronin |
>500 | ZO-1, occludin, Na-K ATPase, pigmentation, TJ complexes, microvilli, basal infold |
D-MEM; Dulbecco’s Modified Eagle Medium, ZO-1; Zonula occludens-1, CRALBP; cellular retinaldehyde-binding protein, N/A; not applicable