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. Author manuscript; available in PMC: 2009 Oct 2.
Published in final edited form as: Nature. 2009 Feb 25;458(7238):651–654. doi: 10.1038/nature07753

Figure 1. Cell autonomous FGF signaling in Kupffer’s Vesicle controls Left-Right patterning.

Figure 1

(a-b) Dorsal view of left-sided spaw expression (arrow) in WT, and bilateral expression in fgfr1 MO 18-20 SS embryos. (c) Percentages of normal (left-sided), reversed, bilateral and absent spaw in WT (n=99), fgfr1 MO (n=117) and fgfr1 MO2 (n=120). (d-e) fgfr1 expression in WT 6 SS embryos. (d) Lateral view (anterior-left) showing fgfr1 expression in KV (bracket) and midbrain-hindbrain (red arrowhead). (e) Tailbud showing fgfr1 expression in KV (white arrow, dorsal view), presomitic mesoderm (red arrowhead) and lateral plate mesoderm (black arrow). (f-g) spaw expression (arrows) in DFCControl MO and DFCfgfr1 MO at 18-20 SS. (h) Percentages of spaw expression in DFC and Yolk MO injected embryos. spaw was altered in DFCfgfr1 MO (n=69) versus DFCControl MO (p<1.19e-05; n=121), with no difference between YolkControl MO (n= 57) and Yolk fgfr1 MO (p<0.90; n= 59).