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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: J Autoimmun. 2009 Mar 26;32(3-4):149–157. doi: 10.1016/j.jaut.2009.02.009

Figure 2. Vκ gene usage.

Figure 2

Splenocytes were pooled from three 5 month-old R4A mice or R4A × FcγRIIB-/- BALB/c mice and stained with fluorochrome-labeled B220 and IgG2b to identify Tg+ B cells. Tg+ B cells were individually sorted into the wells of microtiter plates and PCR was performed on genomic DNA to amplify a partial L fragment spanning FR3 and Jκ regions. Sequence analysis was performed and the Vκ gene families were determined (also presented in Supplemental Table I). The data are shown as the percentage of L chains belonging to each Vκ family.