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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: J Autoimmun. 2009 Mar 26;32(3-4):149–157. doi: 10.1016/j.jaut.2009.02.009

Figure 6. Induction of IL-6 and IL-10 by serum treatment of DCs.

Figure 6

Bone marrow-derived DCs were generated from WT mice (n = 4) or R4A × FcγRIIB-/- mice (n = 4) and treated overnight with 1.0% pooled sera from 10 R4A mice or from 11 R4A × FcγRIIB-/- mice. Induction of IL-6 and IL-10 was measured by qPCR and ELISA. A) Treatment of either WT or FcγRIIB-/- (KO) DCs with R4A × FcγRIIB-/- sera significantly induced IL-6 mRNA and protein (* and ** p < 0.03). B) Treatment with R4A × FcγRIIB-/- sera induced a significant increase in IL-10 mRNA only in WT DCs (*p < 0.03). IL-10 secretion was increased by treatment with R4A × FcγRIIB-/- sera only in WT DCs, but was not significant when compared to treatment with R4A sera, but showed a trend towards being increased. (**p = 0.057). There was no induction of IL-10 when the FcγRIIB-/- DCs were treated with R4A × FcγRIIB-/- sera. Statistical significance was determined by Mann-Whitney. Data are shown as the mean ± SD.