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. Author manuscript; available in PMC: 2009 Sep 5.
Published in final edited form as: J Mol Biol. 2008 Jun 5;381(3):559–568. doi: 10.1016/j.jmb.2008.05.076

Figure 4. Determination of steady state level of TNFα, IL-1β and IL-6 in Myo-3M mice hearts.

Figure 4

Total RNA was extracted from hearts of 24-week old WT/3M, Myo-Tg and Myo-3M mice. mRNA expression was determined using (A) TNFα, (B) IL-1β and (C) IL-6 oligonucleotide labeled with 32P-dATP as a probe. (D) 18S rRNA probe was used as a loading control. Results are presented as the mean SEM and represent three different mice (p < 0.001 compared with the Myo-Tg mice).