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. 2008 Dec 17;28(51):13952–13956. doi: 10.1523/JNEUROSCI.4986-08.2008

Figure 1.

Figure 1.

Gαs*str mice showed striatally enriched transgene expression and cAMP elevation restricted to the striatum. A, The tetracycline-regulated transgenic system used herein employs the CaMKIIα promoter to restrict expression of the transcription factor tTA to postnatal forebrain neurons. The tTA protein then binds the Tet promoter, activating transcription of the ligated transgene. Doxycycline impedes the ability of tTA to bind, thus preventing transcription. As such, the tetracycline system enables expression of a transgene to be suppressed. B, Autoradiographs for CaMKIIα-Gαs* mice showed expression of the transgene throughout postnatal forebrain neurons (nonreversible). C, Autoradiographs for CaMKIIα-tTA/tetO-Gαs* mice showed transgene expression enriched in the striatum (thus, referred to as Gαs*str mice). D, Autoradiographs also showed that transgene expression within Gαs*str mice is reversible with dox, as shown in a Gαs*str-dev mouse (i.e., a mouse that expressed the transgene throughout development but received dox during adulthood). E, As previously measured in CaMKIIα-Gαs* mice (Kelly et al., 2007), Gαs*str mice showed significantly elevated cAMP levels (%CT pmol/mg protein) in the striatum. Gαs*str showed no significant differences in hippocampal or cortical cAMP levels. F, The elevated striatal cAMP levels observed in adult Gαs*str mice were not due to developmental effects, as evidenced by normal cAMP levels in Gαs*str-dev mice. The asterisk (*) versus CT, p = 0.009. FCx, Frontal cortex; Hipp, hippocampus; PCx, parahippocampal cortex; Str, striatum.