Figure 7. Requirements of IL-7R for Vβ8.2 gene germline transcription.

(A) Representative dot plots of DN thymocytes of 4–6 wk-old IL-7Rα^+/+ and IL-7Rα^−/− mice. (B) Percentage of hCD2⁺cells in DN1 fraction shown in (A). (C) Percentage of hCD2⁺ cells in DN2&3 fraction shown in (A). (D) Scatter plot shows the MFI of hCD2 staining in total DN2&3 thymocytes from IL-7Rα^+/+, IL-7Rα^+/− and IL-7Rα^−/− mice. Each dot represents an individual animal of indicated genotype. (E)–(H) Analysis of hCD2 expression in DN thymocytes of ${\mathrm{\gamma }}_{\mathrm{c}}^{+/+}$, ${\mathrm{\gamma }}_{\mathrm{c}}^{+/-}$ and ${\mathrm{\gamma }}_{\mathrm{c}}^{-/-}$ mice. Data are presented in the same format as in (A)–(D). hCD2 expression in DN1 and DN2&3 thymocytes isolated from Vβ8.2^+/+ (shaded area), ${\mathrm{\gamma }}_{\mathrm{c}}^{+/+}$ Vβ8.2^CD2/+ (thin line) or ${\mathrm{\gamma }}_{\mathrm{c}}^{-/-}$ Vβ8.2^CD2/+ (thick line) mice is shown in overlay histogram; 6–8 thymi of the same genotype were pulled together for flow cytometry analysis.