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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: Thromb Haemost. 2008 Nov;100(5):821–828.

Figure 5. Expression of EGR-1 mRNA and GAPDH mRNA in HEL cells in response to PMA (10 nM).

Figure 5

(A) RT-PCR amplification of EGR-1 mRNA. GAPDH was amplified as an internal control. (B) Effect of antisense oligonucleotide to EGR-1 and unrelated oligonucleotide on EGR-1 and Gαq mRNA in PMA-treated HEL cells. The bars represent densitometric analysis. Gαq levels were normalized with GAPDH levels and presented as fold expression relative to levels in the presence of unrelated antisense oligo alone. (C) Effect of anti-EGR-1 oligonucleotide and unrelated antisense oligonucleotide on reporter promoter activity (construct −1042/−1) in HEL cells. Reporter activity was decreased by EGR-1 oligo in a dose dependent manner.