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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: Biochemistry. 2006 Jan 10;45(1):167–174. doi: 10.1021/bi051729t

Figure 2.

Figure 2

Gαi/13 or Gαi/12 chimera interact with RH domains in an activation-dependent manner. GST-p115-RH or GST-LARG-RH were incubated with purified His6-Gαi/13 (upper panels) or His6-Gαi/12 (lower panels), either in the absence or presence of AlF4−, and then GST-RH was pulled down using glutathione Sepharose 4B resin. Protein eluted from washed beads was resolved by SDS-PAGE and either immunoblotted (IB) with anti-Gα13 (B860) or anti-Gα12 (J168) antibody, or stained by Coomassie Brilliant Blue (CBB).